Adaptation of Class-13 -Amylases to Diverse Living Conditions

نویسندگان

  • Anni Linden
  • Matthias Wilmanns
چکیده

There are currently 35 available nonredundant molecular structures of class-13 -amylases (EC 3.2.1.1), mostly from microbial organisms living under a wide range of environmental conditions. One of the most recent additions has been the first -amylase structure of a hyperthermophilic archaeon [Linden et al. , J. Biol. Chem. 2003, 278, 9875 ± 9884] . The structure has been used for comparative analyses with a representative set of three -amylases from thermophilic, mesophilic and psychrophilic sources to identify molecular parameters for environmental adaptation. Our analysis supports generally observed trends such as an increase in structural compactness as well as an increase in salt bridges in order to cope with high-temperature conditions. The two representative thermophilic structures used in this comparative study have independently evolved di-metal centres–not present in the mesophilic and psychrophilic structures–in the vicinity of the active site. These observations may provide impetus for the design of -amylases with improved molecular properties to enhance their utility in biotechnological processes.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Knowledge, Attitude and Practices Regarding Extreme Environments and Cold Adaptation at Extreme Altitudes on the Himalayan Ranges

Introduction: Extreme-altitudes (5500 m/18045 ft and higher) pose environmental, psychophysiological, infrastructural, logistic, and ergonomic challenges that question explorer’s adaptability and mission-efficiency due to isolation, monotony, intimidating environment and terse health conditions. The assessment of an explorer’s comprehensive adaptability in extreme-altitudes is ...

متن کامل

Protein engineering of bacterial K-amylases

K-Amylases constitute a very diverse family of glycosyl hydrolases that cleave K1C4 linkages in amylose and related polymers. Recent structural and mutagenic studies of archeael, mammalian and bacterial K-amylases have resulted in a wealth of information on the catalytic mechanism and on the structural features of this enzyme class. Because of their high thermo-stability, the Bacillus K-amylase...

متن کامل

The role of environments with extreme ecological conditions in the reductive evolutionary development processes of animal

Different groups of animals show phenotypic characters, which have been resulted by the reductive phenomena. The examples are the absence of pigmentation; dwindle of eyes in some cave-living animals, and also the absence of scale in some fishes. These characters are often leaded to evolution of new species with special adaptation that is so called "Regressive evolution". The reductive phenomena...

متن کامل

Phylogenomic Relationships between Amylolytic Enzymes from 85 Strains of Fungi

Fungal amylolytic enzymes, including α-amylase, gluocoamylase and α-glucosidase, have been extensively exploited in diverse industrial applications such as high fructose syrup production, paper making, food processing and ethanol production. In this paper, amylolytic genes of 85 strains of fungi from the phyla Ascomycota, Basidiomycota, Chytridiomycota and Zygomycota were annotated on the genom...

متن کامل

A Mathematical Optimization Model for Scheduling Student Classes in Corona-Living Conditions

Due to the widespread outbreak of Covid-19, many of the world's top universities have decided to hold the next semester virtually. In our country, too, the forecasts show a red situation for some areas in terms of corona prevalence. Due to the large number of students living in these areas, planning for the next semester of universities should be more focused on e-education. According to the in...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2004